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Olympus
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Nikon
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Boston Industries Inc
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Ludl Electronic
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KeyMed Ltd
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TOPTICA Photonics
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ABBELIGHT
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Visitech Inc
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Olympus
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Olympus
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Olympus
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Image Search Results
Journal: International Journal of Nanomedicine
Article Title: Methodological and Cellular Factors Affecting the Magnitude of Breast Cancer and Normal Cell Radiosensitization Using Gold Nanoparticles
doi: 10.2147/IJN.S412458
Figure Lengend Snippet: The mitochondrial membrane potential (JC-1) after the incubation of MDA-MB-231 cells with RGD-PEG 800 -AuNPs 10nm , RGD-PEG 2000 -AuNPs 10nm , RGD-PEG 800 -AuNPs 30nm and RGD-PEG 2000 -AuNPs 30nm . In living cells, the JC-1 dye is absorbed into active mitochondria, where it forms aggregates with fluorescent properties. JC-1 monomers fluoresce under green light (530nm light emission) and JC-1 aggregates fluoresce under red light (595nm light emission). In apoptotic or damaged cells with a low potential of the mitochondrial membrane, JC-1 does not form aggregates, it remains in the form of green fluorescent monomers. The JC-1 level was checked every 5 min till 30 min ( A ). The control group (CTR) was TP of 0 min ( B ). The immunofluorescence JC-1 images were taken after 5, 15 and 25 min of the incubation with AuNPs. The MDA-MB-231 cells were photographed after the 5 min ( C ), 15 min ( D ) and 25 min ( E ) incubation with RGD-PEG 800 -AuNPs 10nm , after the 5 min ( F ), 15 min ( G ) and 25 min ( H ) incubation with RGD-PEG 2000 -AuNPs 10nm , after the 5 min ( I ), 15 min ( J ) and 25 min ( K ) incubation with RGD-PEG 800 -AuNPs 30nm , after the 5 min ( L ), 15 min ( M ) and 25 min ( N ) incubation with RGD-PEG 2000 -AuNPs 30nm . Immunofluorescence was photographed using a Olympus IX83 microscope (Boston Industries, Inc., MA, USA).
Article Snippet: Immunofluorescence was photographed using a
Techniques: Membrane, Incubation, Control, Immunofluorescence, Microscopy
Journal: International Journal of Nanomedicine
Article Title: Methodological and Cellular Factors Affecting the Magnitude of Breast Cancer and Normal Cell Radiosensitization Using Gold Nanoparticles
doi: 10.2147/IJN.S412458
Figure Lengend Snippet: The mitochondrial membrane potential (JC-1) after the incubation of MCF12A cells with RGD-PEG 800 -AuNPs 10nm , RGD-PEG 2000 -AuNPs 10nm , RGD-PEG 800 -AuNPs 30nm , and RGD-PEG 2000 -AuNPs 30nm . In living cells, the JC-1 dye is absorbed into active mitochondria, where it forms aggregates with fluorescent properties. JC-1 monomers fluoresce under green light (530nm light emission) and JC-1 aggregates fluoresce under red light (595nm light emission). In apoptotic or damaged cells with a low potential of the mitochondrial membrane, JC-1 does not form aggregates, it remains in the form of green fluorescent monomers. The JC-1 level was checked every 5 min till 30 min ( A ). The control group (CTR) was TP of 0 min ( B ). The immunofluorescence JC-1 images were taken after 5, 15 and 25 min of the incubation with AuNPs. The MCF12A cells were photographed after the 5 min ( C ), 15 min ( D ) and 25 min ( E ) incubation with RGD-PEG 800 -AuNPs 10nm , after the 5 min ( F ), 15 min ( G ) and 25 min ( H ) incubation with RGD-PEG 2000 -AuNPs 10nm , after the 5 min ( I ), 15 min ( J ) and 25 min ( K ) incubation with RGD-PEG 800 -AuNPs 30nm , after the 5 min ( L ), 15 min ( M ) and 25 min ( N ) incubation with RGD-PEG 2000 -AuNPs 30nm . Immunofluorescence was photographed using a Olympus IX83 microscope (Boston Industries, Inc., MA, USA).
Article Snippet: Immunofluorescence was photographed using a
Techniques: Membrane, Incubation, Control, Immunofluorescence, Microscopy
Journal: International Journal of Nanomedicine
Article Title: Methodological and Cellular Factors Affecting the Magnitude of Breast Cancer and Normal Cell Radiosensitization Using Gold Nanoparticles
doi: 10.2147/IJN.S412458
Figure Lengend Snippet: Immunofluorescence of MDA-MB-231 cells after irradiation and previous incubation with RGD-PEG 800 -AuNPs 10nm , RGD-PEG 2000 -AuNPs 10nm , RGD-PEG 800 -AuNPs 30nm and RGD-PEG 2000 -AuNPs 30nm . The concentration of AuNPs was 0.0004 mg/mL. Three different TPs of the cells incubation with AuNPs before irradiation were used (30 min, 3 and 24 h). Cells after being exposed to AuNPs and irradiated, were analyzed using immunofluorescence to detect γH2AX (red color) and P53 foci (green color). The nuclei of cells were dyed with DAPI solution (blue color). The control group was not exposed to AuNPs and IR ( A – C ). To compare the effect of AuNPs added before IR, the group of cells exposed only to 2 Gy was presented ( D – F ). Images show obtained results of MDA-MB-231 cells after exposition of 2 Gy and: the 30 min ( G ), 3 h ( H ), 24 h ( I ) previous incubation with RGD-PEG 800 -AuNPs 10nm ; the 30 min ( J ), 3 h ( K ), 24 h ( L ) previous incubation with RGD-PEG 2000 -AuNPs 10nm ; the 30 min ( M ), 3 h ( N ), 24 h ( O ) previous incubation with RGD-PEG 800 -AuNPs 30nm and the 30 min ( P ), 3 h ( R ), 24 h ( S ) previous incubation with RGD-PEG 2000 -AuNPs 30nm . Immunofluorescence was photographed using a Olympus IX83 microscope (Boston Industries, Inc., MA, USA).
Article Snippet: Immunofluorescence was photographed using a
Techniques: Immunofluorescence, Irradiation, Incubation, Concentration Assay, Control, Microscopy
Journal: International Journal of Nanomedicine
Article Title: Methodological and Cellular Factors Affecting the Magnitude of Breast Cancer and Normal Cell Radiosensitization Using Gold Nanoparticles
doi: 10.2147/IJN.S412458
Figure Lengend Snippet: Immunofluorescence of MCF12A cells after irradiation and previous incubation with RGD-PEG 800 -AuNPs 10nm , RGD-PEG 2000 -AuNPs 10nm , RGD-PEG 800 -AuNPs 30nm and RGD-PEG 2000 -AuNPs 30nm . The concentration of AuNPs was 0.0004 mg/mL. Three different TPs of the cells incubation with AuNPs before irradiation were used (30 min, 3 and 24 h). Cells after exposition to AuNPs and IR were analyzed using immunofluorescence to detect γH2AX (red color) and P53 foci (green color). The nuclei of cells were dyed with DAPI solution (blue color). The control group was not exposed to AuNPs and IR ( A – C ). To compare the effect of AuNPs added before IR, the group of cells exposed only to 2 Gy was presented ( D – F ). Images show obtained results of MCF12A cells after exposition of 2 Gy and: the 30 min ( G ), 3 h ( H ), 24 h ( I ) previous incubation with RGD-PEG 800 -AuNPs 10nm ; the 30 min ( J ), 3 h ( K ), 24 h ( L ) previous incubation with RGD-PEG 2000 -AuNPs 10nm ; the 30 min ( M ), 3 h ( N ), 24 h ( O ) previous incubation with RGD-PEG 800 -AuNPs 30nm and the 30 min ( P ), 3 h ( R ), 24 h ( S ) previous incubation with RGD-PEG 2000 -AuNPs 30nm . Immunofluorescence was photographed using a Olympus IX83 microscope (Boston Industries, Inc., MA, USA).
Article Snippet: Immunofluorescence was photographed using a
Techniques: Immunofluorescence, Irradiation, Incubation, Concentration Assay, Control, Microscopy